Effect of different light intensities on the growth of the diatom Chaetoceros calcitrans

Batch cultures of C. calcitrans were maintained indoors at a temperature range of 21 to 25 C and continuously illuminated by 40-watt daylight fluorescent lights. Cultures were exposed to 5 different intensities from 200 to 25,000 lux.Population counts show that light intensity affects growth and reproduction of the algae cultivated. A comparison of population peak growths showed cultures illuminated by 12,000 lux to have higher cell counts than those exposed to higher or lower light intensities.

The study of maturational progress in the whitespotted rabbitfish (Siganus sutor) under different photoperiod and light intensity conditions

The rule of light on the timing of maturation and spawning in tropical and subtropical regions is not clear well, because the reproductive cycle in these systems is lunar synchronized. In this study, thus, the effects of different light regimes were investigated on maturational progress of whitespotted rabbitfish, Siganus sutor, the commercial species in Persian Gulf and Oman Sea. During prespawning season, 50 adult fish were randomly divided into ten 300-l tanks (n=5). The fish in control tank received indoor light condition and the fish in each other tanks were exposed to nine different combinations of photoperiod (8L: 16D, 12L: 12D, 16L: 8D) and light intensity (1000, 2000, 3000 lux). After 60 days, GSI and HSI values, serum levels of estradiol (E2), testosterone (T), 17-α hydroxyprogestrone (OHP), calcium (Ca2+) and gonad histology were evaluated for females and males. In females the GSI mean values of exposed fish increased in comparison with control except for fish were kept under 8L, 2000 lux (tank 8). These differences were significant only for fishes in tank 7 (8L, 3000 lux). In the cases of HSI, the results were converse, so that, the most of thanks showed significant decreasing than fishes reared under indoor condition. Morphology and histology study of Ovaries showed three developmental phases including 3, 4 and chiefly 5 that were parallel with GSI values. Fortheremore the serum levels of E2 was recorded between 0.54-15.8 ng/ml in different fish and their mean values were lower than control in all treatmants (P> 0.05). In males, the similar results were obtained. The GSI and HSI mean values in experimental regimes compared with control were upper and lower, respectively, except for fishes were reared in tank 1 (16L, 3000 lux). Testes histology of fishes were reared under different regimes showed signs of stage 5, since no blood vessels observed and thick milt exuded on slight pressure. The mean values of testosterone consentration in fishes were kept in tanks 1 and 6 (12L, 1000 lux) were higher and in other ones were lower than control group. It is also noted that the OHP and Ca2+ had diverse results including increasing and decreasing mean values than control. So, these factors contrary to E2 and T were not suitable to evaluation of maturity in both sexes. On the basis of ovarian structure in stage 5, oocyte development pattern in this species was group synchronous. So, increased mean of GSI versus decreased values of HSI, E2 and perhaps Ca2+ were the signs of improved maturation. But in males, reduced levels of T and similarity of testes morphology in all samples caused that GSI mean value was the only indicator for differentiating among treatments. These findings suggest that alternations were used in light regimes have been the reason of improved maturity in all treatments except fishes reared in tank 8. The ١٠٧ rule of light intensity on induction of maturity was cleared by comparision between fishes in tanks 4, 5, 6 and control group. Because day length was the same whereas fishes in tanks 4, 5 and 6 were exposed to increased light intensity compared with control. This fact verified by results was obtained from fishes in tanks 9 and especially 7, since photoperiod in this group was lower than control. So, higher intensity was considered as the reason of alternations. Contrasting with indoor condition, Induced maturity was also cleared for fishes were kept in tanks 1, 2 and 3, where both of light duration and intensity were increased. But, the rule of photoperiod was individually demonstrated when obtained results were compared with similar light intensities in other treatments. In conclusion, with comparison among different light regimes it is declared that siganids were kept under light condition of tank 2 including 16h light duration combined with 2000 lux intensity showed the best signs of sexual maturation in both sexes. On the basis of this study, setting up the spring light condition during prespawning season induces maturation in withspotted rabbitfish. This improvement not only by influence of photoperiod or light intensity, separately, but obtained through interaction between them. Thus, determination of threshold and resistance to light be recommended before exposure, although using proper regime during suitable time are necessary to achieve purposes considerably.

Physiological observations on a diatom Skeletonema costatum (Greville) Cleve

A chain-forming diatom Skeletonema costatum (Greville) Cleve collected from Yatsushiro Sea, Japan was cultured to determine the optimum level of some physico-chemical factors for their growth under laboratory conditions. Filtered and sterilized aged sea water enriched by adding nutrient solution (Provasoli 1968) was used as the culture medium. The plankton could tolerate a wide range of salinities (3-55 ppt). Optimum growth was observed at salinities of 20-35 ppt, temperatures of 20-25°C, light intensities of 80-120µE mˉ² secˉ¹ and pH between 7.5 and 8.0. Growth did not occur at salinities below 3 ppt and at temperatures above 30°C. From the present study, it is concluded that S. costatum was extremely euryhaline and tolerable to very low salinities.

Studies on cultural conditions of marine Chromatium buderi Truper and Jannasch 1968

Photosynthetic characteristics of a purple sulfur bacterium, Chromatium buderi, cultured under different ranges of pH, temperature, light intensities and ammonium chloride concentrations were examined. Maximum bacteriochlorophyll a synthesis was observed at pH 6.5 whereas the optimum growth was at pH 8.0. In general, higher temperature tended to inhibit the chlorophyll a synthesis and growth. 30°C is the optimum temperature both for chlorophyll a synthesis and growth. At 25µE mˉ²Sˉ¹ the bacteriochlorophyll a content and growth attained maximum level. The response to this low light intensity is an adaptation that ensures a high photosynthetic rate for the purple sulfur bacterium that usually occurs in dimly lit environment. Besides these, ammonium chloride at low concentration enhances both chlorophyll a synthesis and growth. Above 0.5% of it causes the nitrogen-chlorosis and also retards the growth of the bacterium. Possible chemical and structural mechanisms involved are discussed.

Light Reflectance Characteristics and Remote Sensing of Waterlettuce

Waterlettuce ( Pistia stratiotes L.) is a free-floating exotic aquatic weed that often invades and clogs waterways in the southeastern United States. A study was conducted to evaluate the potential of using remote sensing technology to distinguish infestations of waterlettuce in Texas waterways. Field reflectance measurements showed that waterlettuce had higher visible green reflectance than associated plant species. Waterlettuce could be detected in both aerial color- infrared (CIR) photography and videography where it had light pink to pinkish-white image tonal responses. Computer analysis of CIR photographic and videographic images had overall accuracy assessments of 86% and 84%, respectively. (PDF contains 6 pages.)

Analysis of growth layers in the teeth of Tursiops truncatus using light microscopy, microradiography, and SEM

Preliminary results show microradiography and scanning electron microscopy (SEM) to be more accurate methods of accessing growth layer groups (GLGs) in the teeth of Tursiops truncatus than transmitted light microscopy. Microradiography shows the rhythmic deposition of mineral as alternating radiopaque and radiolucent layers. It improves the resolution of GLGs near the pulp cavity in older individuals, better than either SEM or light microscopy. SEM of etched sections show GLGs as ridges and grooves which are easily counted from the micrograph. SEM also shows GLGs to be composed of fine incremental layers of uniform size and number which may allow for more precise age determination. Accessory layers are usually hypomineralized layers within the hypermineralized layer of the GLG and are more readily distinguishable as such in SEM of etched sections and microradiographs than in thin sections viewed under transmitted light. The neonatal line is hypomineralized, appearing translucent under transmitted light, radiolucent in a microradiograph, and as a ridge in SEM. (PDF contains 6 pages.)

Behavioral and Physiological Responses to Light by the Sea Anemone Anthopleura elegantissima as Related to its Algal Endosymbionts

The endosymbiosis of algae with invertebrates may be viewed with at least two major orientations. On the one hand, one may focus on the plant and animal as essentially separate organisms living together, as the word symbiosis states. The products which are exchanged between the plant and animal and the effects of the association on either partner are then of particular interest. On the other hand, one may consider the partnership as an entity, and attempt to investigate the physiology, behavior, etc. of the symbiotic association, observing what differences may appear between the "plant-animal" and analogous non-symbiotic organisms. It is the second approach which I have tried to take in this thesis. I have concentrated on some effects of light on symbiotic and aposymbiotic sea anemones of the species Anthopleura elegantissima, particularly with respect to pigmentation and several types of behavior.

Potentials for fisheries in the Niger Delta: Another green light for self sufficiency in regional food production

A discussion is presented on the potential for fishery development in the Niger Delta region, considering engineering activities and food production potentials of the freshwater zone and immediate hinterland, the brackishwater mangrove swamps and the estuaries. An examination of current trends in the environment indicates that a possible solution to improved exploitation of the region lies in hydraulic engineering, the manipulation of environmental conditions through varying freshwater and seawater inputs so as to increase aquatic and wetland productivity

Preliminary studies on the effects of light duration on the growth and performance of Clarias gariepinus fingerlings

The effects of light duration on the growth and performance of Clarias gariepinus fingerlings were investigated using artificial methods to simulate continuous day length and absolute darkness. The normal day length (12-H Light and 12-H Darkness) served as the control. Among some of the factors affected by the varying photoperiods there were body coloration, feeding efficiency, survival rate and Specific Growth Rate (SGR). There was notably no significant difference between the SGR of the 0-photoperiod culture and the control (P>0.05) but there was significant difference between the 0-photoperiod and the 24-H photoperiod experiment (P<0.05). The haematological profile analysed showed various degrees of changes in the blood parameters of fish cultured under different photoperiods. These changes however, did not show significant differences when subjected to statistical analysis

Reaction of fish to light (”Conclusions” only). [Translation from: Voprosy Ikhtiologii 6 17-20, 1956]

Qualitative and quantitative characteristics of the reaction of certain fish to light were tested and reasons for the attraction of certain fish to light are discussed.

Aspects of mineral composition of male and female Heterobranchus bidorsalis adults exposed to different concentrations of Bonny-light crude oil

Studies were carried out to assess some macro and trace elements of mineral composition of the male and female Heterobranchus bidorsalis adults exposed to graded concentrations (1.00-8.00m/L-1) of Bonny-light crude oil (BLCO). The experiment was monitored for 4 days (toxicity) and 42days (recovery) periods. Significant decreases (P < 0.05)in the sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), phosphorus (P), zinc (Zn), iron (Fe), vanadium (Va), lead (Pb) and manganese (Mn) contents of the male H. bidorsalis corresponded with the increasing concentrations of BLCO. In contrast, the female fishes recorded significant increases (P < 0.05) in the values of the above elements in their tissues as the concentrations of BLCO increased. Furthermore, the values of Na, K, Mg, Ca, P, Zn, Fe, Va, Pb and Mn recorded in the male fishes where generally lower than those of their female counterparts and the control fish. Increased values of these elements were also recorded during the recovery periods (days 14, 28 and 42) of this study in the magnitudes of 15% at day 14, 20% at day 28 and 20% at day 42. This implied that the removal of crude oil stress during this period improved the quantity of these minerals deposited in the fish tissues. The highest percent proportion of Zn and the lowest proportion of Pb recorded in both male and female H. bidorsalis adults agreed with the report of other workers for other fish species. KEYWORDS: Heterobranchus bidorsalis, Mineral composition, Bonny-light crude oil, Toxicity, Recovery.

Effects of rapid decompression and exposure to bright light on visual function in black rockfish (Sebastes melanops) and Pacific halibut (Hippoglossus stenolepis)

Demersal fishes hauled up from depth experience rapid decompression. In physoclists, this can cause overexpansion of the swim bladder and resultant injuries to multiple organs (barotrauma), including severe exophthalmia (“pop-eye”). Before release, fishes can also be subjected to asphyxia and exposure to direct sunlight. Little is known, however, about possible sensory deficits resulting from the events accompanying capture. To address this issue, electroretinography was used to measure the changes in retinal light sensitivity, flicker fusion frequency, and spectral sensitivity in black rockfish (Sebastes melanops) subjected to rapid decompression (from 4 atmospheres absolute [ATA] to 1 ATA) and Pacific halibut (Hippoglossus stenolepis) exposed to 15 minutes of simulated sunlight. Rapid decompression had no measurable influence on retinal function in black rockfish. In contrast, exposure to bright light significantly reduced retinal light sensitivity of Pacific halibut, predominately by affecting the photopigment which absorbs the green wavelengths of light (≈520–580 nm) most strongly. This detriment is likely to have severe consequences for postrelease foraging success in green-wavelength-dominated coastal waters. The visual system of Pacific halibut has characteristics typical of species adapted to low light environments, and these characteristics may underlie their vulnerability to injury from exposure to bright light.

Low cost light traps for coral reef fishery research and sustainable ornamental fisheries

Two relatively inexpensive light traps to capture pre-settling reef fish and invertebrates are described. A trap made from a plastic bucket (with plastic bottles, a small plastic waste bin and two sheets of plywood) that costs US$15 appears to be just as effective as a large aluminium and plexiglass trap that costs US$275.

Effects of current speed and turbidity on stationary light-trap catches of larval and juvenile fishes

Light traps are one of a number of different gears used to sample pelagic larval and juvenile fishes. In contrast to conventional towed nets, light traps primarily collect larger size classes, including settlement-size larvae (Choat et al., 1993; Hickford and Schiel, 1999 ; Hernandez and Shaw, 2003), and, therefore, have become important tools for discerning recruitment dynamics (Sponaugle and Cowen, 1996; Wilson, 2001). The relative ease with which multiple synoptic light trap samples can be taken means that larval distribution patterns can be mapped with greater spatial resolution (Doherty, 1987). Light traps are also useful for sampling shallow or structurally complex habitats where towed nets are ineffective or prohibited (Gregory and Powles, 1985; Brogan, 1994; Hernandez and Shaw, 2003).